Identifying metal-binding small biological molecules

Kutengera momwe thupi limakhalira kumathandiza ofufuza kupeza zomangira zitsulo

Ofufuza apanga njira yodziwira tinthu tating'onoting'ono tomwe timamanga ayoni achitsulo.Ma ion zitsulo ndizofunikira mu biology.Koma kudziwa kuti ndi mamolekyu ati, makamaka tinthu tating’onoting’ono, n’kovuta kwambiri.

Kuti alekanitse ma metabolites kuti aunike, njira zodziwika bwino zama metabolomics zimagwiritsa ntchito zosungunulira za organic ndi ma pH otsika, zomwe zingapangitse kuti zitsulo zisagwirizane.Pieter C. Dorrestein wa ku yunivesite ya California ku San Diego ndi ogwira nawo ntchito ankafuna kuti malowa azikhala pamodzi kuti aunike potengera momwe amakhalira m'maselo.Koma akadagwiritsa ntchito momwe thupi limakhalira panthawi yolekanitsa mamolekyu, akadayenera kukonzanso mikhalidwe yolekanitsa pamtundu uliwonse wa thupi lomwe amafuna kuyesa.

M'malo mwake, ochita kafukufukuwo adapanga njira yamagulu awiri yomwe imayambitsa zochitika za thupi pakati pa kupatukana kwachidziwitso cha chromatographic ndi kusanthula kwakukulu kwa spectrometric (Nat. Chem. 2021, DOI: 10.1038 / s41557-021-00803-1).Choyamba, iwo analekanitsa Tingafinye kwachilengedwenso ntchito ochiritsira mkulu-ntchito madzi chromatography.Kenako adasintha pH yamayendedwe omwe amatuluka pagawo la chromatographic kuti atsanzire momwe thupi limakhalira, kuwonjezera ma ion achitsulo, ndikusanthula kusakanikirana ndi ma spectrometry.Anayendetsa kusanthula kawiri kuti apeze mawonekedwe a mamolekyu ang'onoang'ono okhala ndi zitsulo komanso opanda zitsulo.Kuti adziwe kuti ndi mamolekyu ati omwe amamanga zitsulo, adagwiritsa ntchito njira yowerengera yomwe imagwiritsa ntchito mawonekedwe apamwamba kwambiri kuti azindikire kulumikizana pakati pa mawonekedwe amitundu yomangidwa ndi yosamangidwa.

Njira imodzi yopititsira patsogolo kutsanzira zakuthupi, Dorrestein akuti, ingakhale kuwonjezera ma ayoni ambiri monga sodium kapena potaziyamu komanso kutsika kwachitsulo chosangalatsa."Kumakhala kuyesa mpikisano.Idzakuuzani, Chabwino, molekyuyi pansi pazimenezi imakhala ndi mphamvu zambiri zomanga sodium ndi potaziyamu kapena chitsulo chimodzi chokha chomwe mwawonjezera, "akutero Dorrestein."Titha kuyika zitsulo zambiri nthawi imodzi, ndipo titha kumvetsetsa zomwe amakonda komanso kusankha pankhaniyi."

Muzotulutsa zachikhalidwe kuchokera ku Escherichia coli, ofufuzawo adapeza mankhwala omwe amadziwika kuti amamanga chitsulo monga yersiniabactin ndi aerobactin.Pankhani ya yersiniabactin, adapeza kuti imathanso kumanga zinki.

Ofufuzawo adazindikira kuti zinthu zomwe zimamanga zitsulo m'zitsanzo zimakhala zovuta kwambiri monga momwe zimasungunuka m'nyanja."Ichi ndi chimodzi mwa zitsanzo zovuta kwambiri zomwe ndidaziwonapo," akutero Dorrestein."N'kutheka kuti ndizovuta kwambiri, ngati sizovuta kwambiri, monga mafuta amafuta."Njirayi idazindikira kuti domoic acid ndi molekyu yomanga mkuwa ndipo idati imamanga Cu2+ ngati dimer.

"Njira yodziwika bwino yodziwira ma metabolites onse omanga zitsulo m'chitsanzo ndi yothandiza kwambiri chifukwa cha kufunikira kwa chelation yachitsulo," Oliver Baars, yemwe amaphunzira za metabolites zomanga zitsulo zopangidwa ndi zomera ndi tizilombo toyambitsa matenda ku North Carolina State University. imelo.

"Dorrestein ndi ogwira nawo ntchito amapereka chithunzithunzi chowoneka bwino, chofunikira kwambiri kuti afufuze bwino momwe ma ion achitsulo amagwirira ntchito m'selo," alemba Albert JR Heck, mpainiya wofufuza zakuthambo ku Utrecht University."Chotsatira chotheka chingakhale kuchotsa ma metabolites m'malo omwe amakhala m'maselo ndikugawa nawonso m'mikhalidwe yawo, kuti muwone kuti ndi ma metabolites ati omwe amanyamula ma ayoni achitsulo."

Nthawi yotumiza: Dec-23-2021